Journal: Viruses

Article Title: Co-Deletion of A238L and EP402R Genes from a Genotype IX African Swine Fever Virus Results in Partial Attenuation and Protection in Swine

doi: 10.3390/v14092024

Figure Lengend Snippet: ASFV-specific antibody and cellular immune responses: percentage of blocking by p72-specific serum antibodies after mock-immunisation with PBS ( A ) or immunisation with ASFV-Ke-∆EP402R∆A238L ( B ) determined using a commercial blocking ELISA. Mean+/−SEM of percentage blocking in PBS- or ASFV-Ke-∆EP402R∆A238L-immunised groups ( C ). IFNγ ELISPOT using PBMC isolated from animals at day 28 after immunisation with PBS (blue) or ASFV-Ke-∆EP402R∆A238L (red) and stimulation with either medium or ASFV-Ke wild type (wt) infection at an MOI of 0.1 ( D ).

Article Snippet: The spots were counted using the AID classic ELISPOT reader (AID AutoImmun Diagnostika GmbH.

Techniques: Blocking Assay, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Isolation, Infection

Journal: Cancers

Article Title: A Personalized Neoantigen Vaccine in Combination with Platinum-Based Chemotherapy Induces a T-Cell Response Coinciding with a Complete Response in Endometrial Carcinoma

doi: 10.3390/cancers13225801

Figure Lengend Snippet: Immune profiling of vaccine T-cell response. ( A ) Heatmap representation of relative frequencies of ten main cell subsets in peripheral blood over the DC vaccine program period. ( B ) Vaccine-specific T-cell responses (IFNγ-ELISpot) of PBMCs at baseline (BSL) and after three vaccinations (post-Vax3) post-IVS performed with the vaccination peptide pool (Vaccine). Background response of unstimulated cells were subtracted and SEB was used as positive control. ( C ) Deconvolution (IFNγ-ELISpot) of the nine individual peptides included in the vaccination peptide pool (vaccine) post-IVS. Post Vax6 PBMC were used. Background signal of unstimulated cells were subtracted and SEB was used as positive control. ( D ) Deconvolution (IFNγ-ELISpot) of ten predicted peptides of the positive responding peptide sub-pool after IVS on PBMCs from time point post Vax6. Background response of unstimulated cells were subtracted and SEB was used as positive control. ( E ) Dose response curve (IFNγ-ELISpot) of identified responding mutated vaccination peptides TGIF2, ALDH1B1 and predicted peptide MYH14 compared to their corresponding wild type peptides. Post Vax6 PBMC were used. ( F ) Representative example of cytokine production of CD4 T-cells after re-challenge with TGIF2 peptide at post Vax6. ( G ) Longitudinal analysis of the number of peptide-specific IFNγ-secreting CD4 T-cells per μl of blood. The light blue area represents the period of chemotherapy from post Vax4 to post Vax7. ( H ) Functional profile of TGIF2-specific CD4 T-cells collected at Vax2 and Vax 6. ( B – D ) Error bars represent the standard deviations.

Article Snippet: Then, 100,000 cells were re-challenged (in duplicate) with peptides for 16–18 h, and then plates were washed according to manufacturer’s instructions and counted with the AID-Spot Robot ELISpot reader (AutoImmun Diagnostika GMBH, Straßberg, Baden-Württemberg, Germany).

Techniques: Enzyme-linked Immunospot, Positive Control, Functional Assay